fluorescence probes Search Results


fluorescent probe  (Cytoskeleton Inc)
90
Cytoskeleton Inc fluorescent probe
Fluorescent Probe, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probe  (MedChemExpress)
94
MedChemExpress fluorescent probe
Fluorescent Probe, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluo4 am  (Lumiprobe)
94
Lumiprobe fluo4 am
Fluo4 Am, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rediject 2 dg 750 probe  (Revvity)
91
Revvity rediject 2 dg 750 probe
Rediject 2 Dg 750 Probe, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubility enhancer  (Beyotime)
99
Beyotime solubility enhancer
Solubility Enhancer, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent tsa reagents  (Revvity)
90
Revvity fluorescent tsa reagents
Fluorescent Tsa Reagents, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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fluorescence probe cy5  (Carl Zeiss)
90
Carl Zeiss fluorescence probe cy5
Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated <t>Cy5</t> (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. <t>Fluorescence</t> intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.
Fluorescence Probe Cy5, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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fluorescent cyanine-3 probe  (Carl Zeiss)
90
Carl Zeiss fluorescent cyanine-3 probe
Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated <t>Cy5</t> (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. <t>Fluorescence</t> intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.
Fluorescent Cyanine 3 Probe, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent-cholesteryl ester probe  (Palo Alto Health Sciences)
90
Palo Alto Health Sciences fluorescent-cholesteryl ester probe
Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated <t>Cy5</t> (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. <t>Fluorescence</t> intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.
Fluorescent Cholesteryl Ester Probe, supplied by Palo Alto Health Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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targeted fluorescent probes  (Sandoz)
90
Sandoz targeted fluorescent probes
Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated <t>Cy5</t> (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. <t>Fluorescence</t> intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.
Targeted Fluorescent Probes, supplied by Sandoz, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dcfh-da active oxygen fluorescence probe solution  (Applygen Technologies)
90
Applygen Technologies dcfh-da active oxygen fluorescence probe solution
Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated <t>Cy5</t> (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. <t>Fluorescence</t> intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.
Dcfh Da Active Oxygen Fluorescence Probe Solution, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2,7-dichlorofluorescein diacetate dcfh-da  (Beijing Solarbio Science)
90
Beijing Solarbio Science 2,7-dichlorofluorescein diacetate dcfh-da
Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated <t>Cy5</t> (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. <t>Fluorescence</t> intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.
2,7 Dichlorofluorescein Diacetate Dcfh Da, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated Cy5 (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. Fluorescence intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.

Journal: bioRxiv

Article Title: Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula x Populus tremuloides

doi: 10.1101/2022.06.08.495362

Figure Lengend Snippet: Immunolocalization with pectin antibodies LM19 (recognizing de-esterified homogalacturonan, HG, (a, c)) and LM20 (recognizing methylesterified HG (b, d)) in 1µm thin cross-sections of control roots without fungus (a, b) and colonized roots (c, d) collected at 14 days after contact (DAC) (or control without fungus). L. bicolor is visualized by labelling with WGA-AF488 (green); and plant cell-wall homogalacturonan is visualized with LM19 and LM20 antibody conjugated Cy5 (magenta). White arrows indicate the epidermal layer where the difference of antibody labelling abundance is evident between ECM and control roots. Scale bar = 20 µm. Insets in images a-d show a magnified view of three adjacent epidermal cells. Fluorescence intensity ratio of LM19 and LM20 in the epidermal layer (e) and whole tissue area (f), respectively, at the indicated time-points after contact. Data was collected from ten observations per root and four to five roots per timepoint and condition. ** p-value <0.01, * p-value <0.05 with the student t-test.

Article Snippet: We indirectly quantified pectin epitopes by measuring the fluorescence probe (Cy5) intensity (mean number of pixels per unit area) conjugated with the respective primary antibody using the ‘curve spline’ function of the Zeiss ZEN blue software.

Techniques: Control, Fluorescence